Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Qualitative analysis of the staining on the cell array treated with the anti-Ep-CAM fluorescein-conjugated mAb. A: Reconstruction of the cell array using high-magnification images after superimposition of the fluorescein (Ep-CAM) and Hoecht (DNA) signal. B: Immunofluorescence staining with the anti-human Ep-CAM fluorescein conjugated mAb on the Hep3b and COLO205 cells. The different cell lines were positioned onto the array as follow: A2, BKGE; A3, COLO205; A4, HCT116; A5, SW620; B1, PC3; B2, DU145; B3, NRP154; B4, HIAEC; B5, HUVEC; C1, HMVEC; C2, NHDF; C3, CASMC; C4, NHEK; C5, A375; D1, SkBr3; D2, MCF7; D3, BT474; D4, A673; D5, HepG2; E1, Hep3b; E2, Jurkat; E3, Bjab; E4, SKMES1; and E5, U87MG.

Article Snippet: Tumor cells included: human colorectal carcinoma cell lines COLO205, SW620, HCT116; human prostate carcinoma cell lines PC3, DU145; human breast carcinoma cell lines SkBr3, MCF7, BT474; human malignant melanoma cell line A375; human rhabdomyosarcoma cell line A673; human neuroglioma cell line U87MG; human lung carcinoma cell line SKMES1; human liver carcinoma cell lines HepG2, Hep3b; T- and B-cell leukemia cell lines Jurkat and Bjab, respectively, were purchased from American Type Culture Collection (Manassas, VA).

Techniques: Staining, Immunofluorescence

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Binding of an anti-human Ep-CAM fluorescein-conjugated mAb to various cell lines analyzed by FACS. BKGEs (A2); human colorectal carcinoma cell lines COLO205 (A3), HCT116 (A4), and SW620 (A5); human prostate carcinoma cell lines PC3 (B1) and DU145 (B2); rat prostate tumorigenic cell line NRP154 (B3); HIAECs (B4); HUVECs (B5); lung HMVECs (C1); NHDFs (C2); human CASMCs (C3); neonatal NHEKs (C4); human malignant melanoma cell line A375 (C5); human breast carcinoma cell lines SkBr3 (D1), MCF7 (D2), and BT474 (D3); human rhabdomyosarcoma cell line A673 (D4); human liver carcinoma cell lines HepG2 (D5) and Hep3b (E1); T- and B-cell leukemia cell lines Jurkat (E2) and Bjab (E3), respectively; human lung carcinoma cell line SKMES1 (E4); and human neuroglioma cell line U87MG (E5) were incubated in the absence (thin line) or presence (thick line) of an anti-human Ep-CAM fluorescein-conjugated monoclonal antibody and analyzed by FACS.

Article Snippet: Tumor cells included: human colorectal carcinoma cell lines COLO205, SW620, HCT116; human prostate carcinoma cell lines PC3, DU145; human breast carcinoma cell lines SkBr3, MCF7, BT474; human malignant melanoma cell line A375; human rhabdomyosarcoma cell line A673; human neuroglioma cell line U87MG; human lung carcinoma cell line SKMES1; human liver carcinoma cell lines HepG2, Hep3b; T- and B-cell leukemia cell lines Jurkat and Bjab, respectively, were purchased from American Type Culture Collection (Manassas, VA).

Techniques: Binding Assay, Incubation

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Capture of the cell array fluorescent signal using different image acquisition systems. A: Hoechst fluorescent staining was analyzed for each spot using the same capture parameters on the fluorescence microscope and separate images were used to rebuild the complete cell array. B: Anti-human Ep-CAM fluorescein-conjugated mAb binding to the cell array analyzed using the same capture parameters on the fluorescence microscope. Separate images were used to rebuild the complete cell array. C and D: Anti-human Ep-CAM fluorescein-conjugated mAb binding to the cell array was analyzed using a Typhoon 8600 scanner or a white-light source CCD camera, respectively. Remarkably, the same array slide was used for the fluorescence microscopy and white-light source CCD camera analysis, whereas a different slide was used for the typhoon 8600 scanner analysis. The different cell lines were positioned onto the array as follows: A2, BKGE; A3, COLO205; A4, HCT116; A5, SW620; B1, PC3; B2, DU145; B3, NRP154; B4, HIAEC; B5, HUVEC; C1, HMVEC; C2, NHDF; C3, CASMC; C4, NHEK; C5, A375; D1, SkBr3; D2, MCF7; D3, BT474; D4, A673; D5, HepG2; E1, Hep3b; E2, Jurkat; E3, Bjab; E4, SKMES1; and E5, U87MG. Original magnifications, ×40.

Article Snippet: Tumor cells included: human colorectal carcinoma cell lines COLO205, SW620, HCT116; human prostate carcinoma cell lines PC3, DU145; human breast carcinoma cell lines SkBr3, MCF7, BT474; human malignant melanoma cell line A375; human rhabdomyosarcoma cell line A673; human neuroglioma cell line U87MG; human lung carcinoma cell line SKMES1; human liver carcinoma cell lines HepG2, Hep3b; T- and B-cell leukemia cell lines Jurkat and Bjab, respectively, were purchased from American Type Culture Collection (Manassas, VA).

Techniques: Staining, Fluorescence, Microscopy, Binding Assay

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Comparison of the FACS and the Cell Array Data Acquired Either with the Microscope Setup, a Typhoon 8600 Scanner, or a White Light Source CCD Camera

Article Snippet: Tumor cells included: human colorectal carcinoma cell lines COLO205, SW620, HCT116; human prostate carcinoma cell lines PC3, DU145; human breast carcinoma cell lines SkBr3, MCF7, BT474; human malignant melanoma cell line A375; human rhabdomyosarcoma cell line A673; human neuroglioma cell line U87MG; human lung carcinoma cell line SKMES1; human liver carcinoma cell lines HepG2, Hep3b; T- and B-cell leukemia cell lines Jurkat and Bjab, respectively, were purchased from American Type Culture Collection (Manassas, VA).

Techniques: Comparison, Microscopy

Journal: BMC Cancer

Article Title: uPAR enhances malignant potential of triple-negative breast cancer by directly interacting with uPA and IGF1R

doi: 10.1186/s12885-016-2663-9

Figure Lengend Snippet: Co-overexpression of uPAS components and tumour-promoting proteins in TNBC samples and cell lines. a Immunohistochemical analysis of tumour samples showing positive expression and localisation of the proteins of interest: uPAR, uPA, PAI-1, IGF1R, IR and c-Met, bar: 50 μm and b Protein expressions of uPAR, uPA, PAI-1, IGF1R, IR and c-Met in the TNBC cohort ( n = 174). c Immunoblottings of uPAR, uPA (supernatant), PAI-1, IGF1R, (phospho) c-Met, HER2, ER, PR in two TNBC cell lines: BT549 and MDA-MB-231 and in the breast cancer cell lines: BT474, MCF7, MDA-MB-361, SKBR3 and T47D. Tubulin was used as loading control

Article Snippet: The following human breast cancer cells lines MDA-MB-361 (HTB-27), SKBR3 (HTB-30), T47D (HTB-133) were acquired from American Type Culture Collection (ATCC) and MCF7 (ACC115) cells from German Collection of Microorganisms and Cell Cultures, DSMZ).

Techniques: Over Expression, Immunohistochemical staining, Expressing, Control